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The undiluted serum samples obtained after centrifugation of clotted blood were deproteinized by filtration through the Amicon Ultra-0.5 Centrifugal Filter Unit, 10 kDa (Merck Millipore, Burlington, USA)

This aids in mitigating accuracy limitations because its representation cannot completely capture the models performance across different operating conditions or when the models behavior changes

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When you consume NAC in the form of food or supplements, your gut absorbs the NAC, transports it to your liver, and the liver converts it to cysteine